Methods

The invention of super-resolution microscopy has revolutionized the study of cell biological processes. It enables the investigation of the localization and distribution of proteins at the sub-cellular level. In particular, the group uses STED microscopy to quantify physiological and pathological processes in cells and tissues. The visualization of fundamental biological relationships on the nanoscale allows the characterization of new pharmacological agents and their testing in new therapeutic procedures in unprecedented detail.

We offer:

• Planning, sample preparation, data acquisition and evaluation for ultrahigh-resolution microscopy

The research group develops and uses intelligent algorithms to automate the process of super-resolution microscopy. The selection of suitable targets on a sample and their acquisition at maximum resolution are performed independently without intervention by the user. Subsequently, the acquired image data is forwarded to an AI-supported data analysis.

We offer:

• Development of autonomous microscopy systems
• Customized, automated image data analysis

The use of a high-throughput microscopy system allows the group to systematically screen compounds for their potential for use as novel pharmacologics.

We offer:

• High-throughput screening of active ingredients 

The research group has many years of experience in the production of genome-edited cell lines. It uses CRISPR/Cas technology to tag target proteins with markers for super-resolution light microscopy. In addition, CRISPR/Cas allows targeted deletion or mutation of genes whose pathological phenotype leads to known central nervous system diseases. The generation of known genetic defects in a cell culture allows the controlled investigation of diseases under laboratory conditions.

We offer:

• Targeted creation of genome-edited cells 

The research group uses all the methods already mentioned for the development and establishment of assays for the comprehensive characterization of new pharmacologically active substances: From the generation of genome-edited cell lines, to automated super-resolution microscopy, to tailored data analysis.

We offer:

• Assay development for comprehensive drug characterization