Methods

Technologies used:

• in vitro cell culture
  • Functional cell tests with mast cells
  • Functional cell tests with basophil granulocytes
  • Cell-cell interaction tests (mast cells:eosinophil granulocytes; mast cells:T cells; mast cell-skin fibroblasts)
• ex vivo skin models
  • Functional cell tests in skin explants
  • Cell-cell interaction tests (e.g. mast cells: eosinophil granulocytes)
  • Drug diffusion test
• Spectral flow cytometry and cell sorting
• Biolayer interferometry
• ELISA
• Multiplex cytokine profiling
• immunohistochemistry
• in situ hybridization
• spatial transcriptomics
• Skin microdialysis
• Microneedle-based ISF extraction
• Biolayer interferometry
• Nanotemper
• Digital PCR using Protein Interaction Coupling (PICO))
• Western blotting
• Organoids (e.g. sweat glands)